Quality control sample of total number of colonies in feed|BNCC361567 |BNCC

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Quality control sample of total number of feed colonies

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	After taking out the quality control sample from the refrigerator at -20 ℃, placing it at room temperature for 30 min, then opening it in the biosafety cabinet, immediately adding 1.1mL sterile water or sterile physiological saline to redissolve the freeze-dried quality control substance, fully mixing and preparing the sample suspension, which is the quality control sample with a concentration of 2.0~2.6 × 10 6CFU/ml.
Growth conditions	37 ℃;18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	2
application	GB/T 13093-2023 Determination of Total Bacteria in Feeds
Sharing mode	Public welfare sharing