Water quality Enzyme substrate method quality control sample (50L water sample)|BNCC361780 |BNCC

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Water quality enzyme substrate method quality control sample

Culture medium	Ammonium sulfate: 5.0, manganese sulfate: 0.0005, zinc sulfate: 0.0005, magnesium sulfate: 0.1, sodium chloride: 10.0, calcium chloride: 0.05, sodium sulfite: 0.04, amphotericin B:0.001,ONPG:0.5,MUG:0.075,Solanium extract: 0.5,HEPES:6.9,HEPES sodium salt: 5.3
Subculture procedure	Samples required a total of 50L of sterile water or sterile saline to dissolve. Before use, place the quality control sample at room temperature for 30min, then open it in the biosafety cabinet, immediately add 5mL of sterile water, suck it out after dissolution and put it into a sterile bottle, repeatedly wash the inner wall of the vial with the remaining sterile water, and transfer it to the above sterile bottle, which is the standard sample stock solution.
Growth conditions	37 ℃, aerobic; 44.5 ℃, aerobic; incubation time 24 hours;
Storage conditions	-20 ℃, dark and refrigerated; ice bag transportation
Safety level	2
application	Reference standard: HJ 1001-2018 Enzyme substrate method for determination of total coliform, fecal coliform and Escherichia coli in water quality; Quality control of enzyme substrate method for detection of total coliform, fecal coliform and Escherichia coli in water quality. The strain numbers required for production are: BNCC269342, BNCC286903 and BNCC238481.
Sharing mode	Public welfare sharing