BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | After taking out the quality control sample from the refrigerator at -20 ℃, placing it at room temperature for 30 min, then opening it in the biosafety cabinet, immediately adding 1.1mL sterile water or sterile physiological saline to redissolve the freeze-dried quality control substance, fully mixing and preparing the sample suspension, which is the quality control sample with a concentration of 0.6-1.2 × 10 5CFU/ml. |
Growth conditions | 37 ℃;18-24h; aerobic; |
Storage conditions | -20 ℃ |
Safety level | 2 |
application | GB/T 13093-2023 Determination of Total Bacteria in Feeds |
Sharing mode | Public welfare sharing |