Quantitative strain of Aspergillus niger|BNCC353778 |BNCC

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Quantitative strain of Aspergillus niger

Culture medium	Potato Glucose Agar (PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	1. According to the arrow sign of penicillin bottle cap, open the strain and dissolved liquid; 2. transfer the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve; 3. After being fully dissolved, it can be absorbed proportionally according to the requirements; 4. Please use it up immediately after opening and do not store it;
Growth conditions	28,5-7d, aerobic
Storage conditions	-20 ℃
morphology	Colony large, dense, hyphae white, margin pseudofilamentous, spreading, producing black spores
Separation substrate	Blueberry, North Carolina
Sharing mode	Public welfare sharing

Aspergillus brasiliensi

strain number: BNCC 353778 / ATCC16404

Name: BNCC 353778 / ATCC16404

Product format: quantitative bacteria tablets

number of live bacteria: 0.5-1.0x10 3CFU/bottle

product batch: 191212 valid until: 2020.12.11

inspection date: 12.16.2019 sampling ratio: ≥2%

report number: RS19353778-1

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Product list: 0.5-1. 0x103CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;

shelf life: unopened, below -20 ℃, 12 months;

quality inspection project	inspection description	result
colony morphology	observe single colony morphology	large colonies, dense, white hyphae, false filamentous edges, spreading and growing, producing black spores
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
reference value of live bacteria	non-selective medium plate, viable bacteria count	980CFU/bottle
phenotype and genotype analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control inspection system, the product is qualified

Handling procedure:

1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 it will be 0.5-1.0x10 3cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Attentions:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization