Quantitative quality control strains of Clostridium sporogenes (plate count method)|BNCC359610 |BNCC

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Quantitative strains of clostridium sporogenes

Culture medium	Special peptone: 23.0, soluble starch: 1.0, sodium chloride: 5.0, agar: 10.0, defibrated sheep blood: 5% (added at 50 ℃),pH:7.3±0.2(25 ℃)
Subculture procedure	① Prepare a test tube and 2 plates containing 5~10mL of liquid culture medium; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then crush it with tweezers; ③ Absorb 0.5mL of liquid culture medium into a freeze-drying tube, fully dissolve it, and then return it to the liquid test tube for mixing. ④ Absorb 0.2mL of bacteria into a suspension plate and coat evenly, repeat twice to obtain two plates; ⑤ Place all the liquid test tubes and plates under the above culture conditions, and the strains can be used when they grow out.
Growth conditions	37 ℃;18-24h; anaerobic;
Growth characteristics	G +, cell straight rod, 0.73 x 1.46~3.33 m. Mostly a single arrangement. Spores ellipsoid, secondary, cyst expanded. After the formation of spores, the bacteria rapidly decompose, leaving only spores.
Storage conditions	-20 ℃
Safety level	2
morphology	The colony is 1-2mm in diameter, round, with neat edges, opaque, white on the front, raised in the middle, bright on the surface, smooth on the surface, moist in texture, easy to pick up, G + (blue-purple), bacilli, purity: pure
application	research; analysis and detection. Teaching and scientific research. "Disinfection Technical Specification" Bacteria for Testing Sterilization Effect of Traditional Chinese Medicine Instruments, "GB/T 4789.12-2003 Clostridium Botulinum and Botulinum Toxin Test" Positive Control Strains, 2015 Edition of Chinese Pharmacopoeia Quality Control Strains. 2020 edition of pharmacopoeia-"general 1100 biological examination method" test strain pharmacopoeia bacteria, medium "sensitivity examination" test strain, "method applicability test" test strain, "medium applicability test and control bacteria test method applicability test" test strain
Sharing mode	Public welfare sharing