BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | Before use, place the quality control sample at room temperature for 30 min, then open it in the biological safety cabinet, immediately add 5mL sterile water or sterile physiological saline to redissolve the freeze-dried quality control material, and after fully mixing, it will be a sample suspension with a concentration of 30 ~ 300CFU/mL. |
Growth conditions | Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic atmosphere; |
Storage conditions | -20 ℃ |
Safety level | 0 |
application | It is applicable to the detection of the total number of colonies, quality control and personnel assessment in the standards of "HJ 1000-2018 Determination of the Total Number of Bacteria in Water Quality" and "GB/T 18204.4-2013 Public Places Health Inspection Methods Part 4: Microorganisms for Public Supplies. |
Sharing mode | Public welfare sharing |