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BNCC > Microbial strain > Aspergillus brasiliensis Varga et al.
Aspergillus brasiliensis Varga et al.-BNCC
  • BNCC
  • Aspergillus brasiliensis Varga et al.-BNCC

Aspergillus brasiliensis Varga et al.

  • Price： $ 286
  • number：BNCC352057
  • Form：
    Filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are white, dense/low, the hyphae are vigorous and spread, and a large number of black spores are produced.
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
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Essential Information Certificate Related Products
Aspergillus brasiliensis Varga et al.
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Growth conditions 28 ℃,5-7 days; Aerobic
Storage conditions 2-8 ℃
morphology Filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are white, dense/low, the hyphae are vigorous and spread, and a large number of black spores are produced.
Separation substrate Blueberry, North Carolina
Sharing mode Public welfare sharing

Aspergillus brasiliensis

Storage conditions : 2~8 ℃

No. 352057

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, 5-7 days, comprehensive PDA agar medium: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates; 
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

Item test results
viability: good viability, in 3 days, floc growth at the top of the culture solution, 5 days strain layer become obvious,  colony is typical
colony morphology: (above)

filamentous fungi have obvious colonies on comprehensive PDA culture medium,

hyphae are white, dense/low, hyphae are flourishing and grow, producing a large number of black spores
Conclusion: good viability, no abnormal colony morphology, qualified

 

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