Staphylococcus aureus quantitative strains|BNCC381462 |BNCC

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Quantitative strains of Staphylococcus aureus

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃;18-24h; aerobic
Growth characteristics	G + cocci, single, paired, irregularly stacked. No sprouts. Ornithine decarboxylase negative, fermentation of glucose, maltose, lactose, sucrose acid production does not produce gas, fermentation of mannitol. Methyl red reaction was positive, V-Pu test was weakly positive. A transparent hemolytic ring was formed around the blood plate colonies. Production of golden yellow pigment. facultative anaerobic, optimum pH7.0-7.5.
Storage conditions	-20 ℃
Safety level	2
morphology	The colony is 1-2mm in diameter, round, with neat edges, opaque, golden yellow on the front, light color, convex in the middle, smooth surface, bright surface, moist texture, easy to stir up, no pigment, G + (blue-purple), cocci, purity: pure
application	It is mainly used for medium performance testing, positive control, laboratory ability verification, personnel ability verification, laboratory quality control and method evaluation.
Sharing mode	Public welfare sharing