Aspergillus brasiliensis Varga et al.|BNCC353552 |BNCC

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Aspergillus brasiliensis Varga et al.

Culture medium	Potato Glucose Agar (PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions	25°C, aerobic
Storage conditions	-20 ℃
morphology	migratory growth, irregular edges, opaque, black on the front, black on the back, flat shape, rough surface, dark surface, dry texture, purity: pure
Separation substrate	Blueberry, North Carolina
Sharing mode	Public welfare sharing

Aspergillus brasiliensis

strain number: BNCC 353552 / ATCC 16404

product format: quantitative bacteria tablets

number of live bacteria : 1.0~2.0 x 10 3CFU/bottle

product batch: 190916

valid until: 3.15. 2020

inspection date: 9.16. 2019

sampling ratio: ≥2%

report number: RS19353552-1

biosafety level: 1, handle in ultra-clean table or safety cabinet

quality inspection project	inspection description	results
colony morphology	observe single colony morphology	hyphae are white, black spores, vigorous
purity	non-selective medium culture, colony observation and microscopic examination	no bacteria
reference value of live bacteria	non-selective medium plate, live bacteria count	1240CFU/bottle
phenotype and genotype analysis	phenotype test, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

product list:1.0~2.0x10 3 CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;

shelf life: unopened, below -20 ℃, 6 months;

Handling procedure:

1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 it will be 1.0~2.0x10 3 cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Attentions:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization

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