Standard substance for plate count of Escherichia coli|BNCC366383 |BNCC

Essential Information Related Products

Reference material of Escherichia coli Count

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃, aerobic
Storage conditions	-20 ℃
Safety level	1
morphology	Light yellow, raised, moist, bright, G-bacillus
Separation substrate	clinical isolates
application	Sensitivity test; Bacteriostasis test; Food detection; Cosmetic detection; Culture control; Quality control strain; API verification bacteria, positive control strain in GB 4789.38-2012 Escherichia coli count.
Sharing mode	Public welfare sharing