Aspergillus niger quantitative strain|BNCC364981 |BNCC

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Quantitative strains of aspergillus niger

Culture medium	Potato Impregnation Powder: 10.0, Glucose: 20.0,KH2PO4:3.0,MgSO4 · 7H2O:1.5, Thiamine: 0.008, Agar: 15.0,pH:6.0±0.2(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	28 ℃;48-72; aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on the integrated PDA medium, dense and vigorous hyphae, and a large number of black spores are produced in the later period and the plates are covered with yellow on the back of the medium.
application	research; analysis and detection. 2015 edition of Chinese Pharmacopoeia quality control strains, GB 4789.15-2010 mold and yeast count positive control strains. Spore-producing.
Sharing mode	Public welfare sharing