Water quality total coliform negative quality control sample (rapid paper method)|BNCC360413 |BNCC

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Water quality total coliform negative quality control sample (paper rapid method)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Negative quality control bacteria:; Activate and increase the bacteria according to the above quality control sample, so that the absorbance of the ten-fold dilution of the two bacteria is in the range of 0.3~0.5; Mix the two kinds of original bacteria liquid in equal volume, dilute 25000 times and then suck 0.5mL of final diluent into 10mL protective agent (quantitative bacteria protective agent), and mix well; Sub-pack the mixed solution into 0.4mL/vial, then place it in -80 ℃ for 20~24 hours, take it out and freeze it overnight, and cap the freeze-dried quality control product and label it for storage the next day;
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, protected from light
Safety level	2
application	Specific uses: quality control and personnel assessment by rapid paper detection of total coliform and fecal coliform in water quality; Applicable standard "HJ 755-2015 Rapid Paper Method for Determination of Total Coliform and Fecal Coliform in Water Quality"
Sharing mode	Public welfare sharing