Quantitative quality control strains of Pseudomonas aeruginosa (enzyme substrate method)|BNCC370599 |BNCC

Essential Information Related Products

Pseudomonas aeruginosa quality control sample

Culture medium	Ammonium sulfate: 5.0, manganese sulfate: 0.0005, zinc sulfate: 0.0005, magnesium sulfate: 0.1, sodium chloride: 10.0, calcium chloride: 0.05, sodium sulfite: 0.04, amphotericin B:0.001,ONPG:0.5,MUG:0.075,Solanium extract: 0.5,HEPES:6.9,HEPES sodium salt: 5.3
Subculture procedure	Before use, place the quality control sample at room temperature for 30min, and the sample needs a total of 500mL of sterile water or sterile physiological saline to dissolve. Then open it in the biosafety cabinet, immediately add 3~4mL of sterile water, suck it out after dissolution and put it into a sterile bottle, repeatedly wash the inner wall of the vial with the remaining sterile water, and transfer it to the above sterile bottle, I .e. 500mL of standard sample stock solution.
Growth conditions	37 ℃; culture time 24-48 hours; gas environment anaerobic
Storage conditions	-20 ℃, protected from light
Safety level	2
application	Reference standard: HJ 1001-2018 Enzyme substrate method for determination of total coliform, fecal coliform and Escherichia coli in water quality; Negative control for determination of total coliform, fecal coliform and Escherichia coli in water quality (enzyme substrate method).
Sharing mode	Public welfare sharing