BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | Samples required a total of 200mL of sterile water or sterile saline to dissolve. Before use, place the quality control sample at room temperature for 30min, then open it in the biosafety cabinet, immediately add 3-4mL of sterile water, suck it out after dissolution, and put it into a sterile bottle, and repeatedly wash the inner wall of the vial with the remaining sterile water. Transfer the whole sample to the above sterile bottle, which is the negative quality control sample, with a total of 200mL and a concentration of 300-3000MPN/L. |
Growth conditions | Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment |
Storage conditions | -20 ℃, protected from light |
Safety level | 1 |
application | It is suitable for the determination of fecal coliform in water quality, quality control and personnel assessment (multi-tube fermentation method). Reference standard "HJ 347.2-2018 water quality-Determination of fecal coliforms-Multi-tube fermentation method" |
Sharing mode | Public welfare sharing |