Fecal coliform standard quality control sample (MPN counting method)|BNCC360589 |BNCC

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Standard quality control sample of fecal coliform (MPN counting method)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Samples required a total of 100mL of sterile water or sterile saline to dissolve. Before use, place the quality control sample at room temperature for 20min, then open it in the biosafety cabinet, immediately add 3-4mL of sterile water, suck it out after dissolution, and put it into a sterile bottle. Repeat washing the inner wall of the vial with the remaining sterile water, and transfer it to the above sterile bottle, which is the standard quality control sample, totaling 100mL, with a concentration of 10-100MPN/mL.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, protected from light
Safety level	2
application	Quality control and personnel assessment of fecal coliform counts in food. Reference standard "GB4789.39-2013 national food safety standard food microbiological detection fecal coliform count" MPN counting method.
Sharing mode	Public welfare sharing