Staphylococcus aureus quantitative strains|BNCC361282 |BNCC

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Quantitative strains of staphylococcus aureus

Culture medium	Beef extract: 3.0, peptone: 10.0,NaCl:5.0, agar: 15.0,pH:7.0(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃,18h, aerobic
Storage conditions	-80 ℃
Safety level	2
morphology	The colony is 0.5-1mm in diameter, round, neat edges, opaque, yellow on the front, convex in the middle, smooth surface, easy to provoke, G + (blue-purple), cocci
application	It is mainly used for medium performance testing, positive control, laboratory ability verification, personnel ability verification, laboratory quality control and method evaluation.
Sharing mode	Public welfare sharing