BeNa Culture Collection
Culture medium | Potato Glucose Agar (English Name: PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L. |
Subculture procedure | (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow. |
Growth conditions | 28 ℃;3-5 days; Aerobic |
Storage conditions | 2-8 ℃ |
Separation substrate | Site for stacking rotten honey pomelo |
application | Synthetic naringinase, pectinase, etc., used for microbial strains of feed additives |
Sharing mode | Public welfare sharing |