Aspergillus aculeatus|BNCC196238 |BNCC

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Aspergillus aculeatus

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions for 5-7 days.
Growth conditions	28 ℃,5-7 days, aerobic
Growth characteristics	Black spores were produced on the CPDA slope. 5-7 days
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on integrated PDA medium, white hyphae at the initial stage, dense and vigorous, and brown spores later.
Separation substrate	Nonigo
Sharing mode	Public welfare sharing

1.Description

1. Name：Aspergillus aculeatus

2. BNCC No.：196238

3. Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

2. Atmosphere：aerobic

3. Temperature：25-28 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization