Standard sample of total number of colonies in cosmetics|BNCC361614 |BNCC

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Standard Sample of Total Colony in Cosmetics

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	After taking out the quality control sample from the refrigerator at -20 ℃, it is placed at room temperature for 30 min, then it is opened in the biosafety cabinet, 1.1mL sterile water or sterile physiological saline is immediately added to redissolve the freeze-dried standard substance, and the sample suspension is prepared after full mixing, which is the standard sample with a concentration of 0.6-1.2 × 10 5CFU/ml.
Growth conditions	37 ℃;18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	2
application	GB/T 7918.2-1987-Standard methods of microbiological examination for cosmetics-Determination of total bacterial count, etc.
Sharing mode	Public welfare sharing