Bacillus atrophy spore suspension|BNCC358969 |BNCC

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Bacillus subtilis spore suspension

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	30 ℃;18-24h; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony is 1-2mm in diameter, round, yellow on the front, light in color, raised in the middle, bright and opaque on the surface, neat edges, smooth surface, moist texture, G (blue-purple), bacilli
application	Resistance Testing Dry heat sterilization control Efficacy testing Ethylene oxide sterilization control Media testing Steam sterilization control Sterility assurance Sterilization control Pharmaceutical and Personal Care resistance test Dry heat sterilization control Efficacy test Ethylene oxide sterilization control Media test BR> Sterility assurance Sterilization control> Pharmaceuticals and personal care
Sharing mode	Public welfare sharing