BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | Samples required a total of 100 mL of sterile water or sterile saline to dissolve. Before use, place the quality control sample at room temperature for 30 min, then open it in the biosafety cabinet, immediately add 3-4 mL of sterile water, dissolve it, suck it out and put it into a sterile bottle, repeatedly wash the inner wall of the vial with the remaining sterile water, and transfer it to the above sterile bottle, which is the standard quality control sample, with a total of 100 mL and a concentration of 300-15000 MPN/L. |
Growth conditions | Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment |
Storage conditions | -20 ℃, protected from light |
Safety level | 2 |
application | Specific uses: quality control and personnel assessment by rapid paper detection of total coliform and fecal coliform in water quality; Applicable standard "HJ 755-2015 Rapid Paper Method for Determination of Total Coliform and Fecal Coliform in Water Quality" |
Sharing mode | Public welfare sharing |