Water quality Fecal coliform standard quality control sample (membrane filter method)|BNCC360398 |BNCC

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Water quality fecal coliform standard quality control sample (Membrane membrane method)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Samples required a total of 100mL of sterile water or sterile saline to dissolve. Before use, place the quality control sample at room temperature for 30min, then open it in the biosafety cabinet, immediately add 3~4mL of sterile water, suck it out after dissolution, and put it into a sterile bottle. The remaining sterile water is repeatedly washed with the inner wall of the vial, and all of it is transferred to the above sterile bottle, which is the standard quality control sample, equivalent to 100mL of water sample to be tested. It is suggested that if the filtration volume is 100mL, the number of fecal coliform colonies is 20-60, and the total number is less than 200.
Growth conditions	Culture temperature 37 ℃; Culture time 18~24 hours; Aerobic gas environment
Storage conditions	-20 ℃, protected from light
Safety level	1
application	It is suitable for quality control and personnel assessment (filter membrane method) for the determination of fecal coliform bacteria in water quality. Reference standard "HJ 347.1-2018 water quality-Determination of fecal coliforms-Membrane method"
Sharing mode	Public welfare sharing