Candida albicans nucleic acid detection kit (fluorescent PCR method)|BNCC361452 |BNCC

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Candida albicans nucleic acid detection kit (QPCR)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Experimental preparation: before the experiment, the sample is cultured with reference to SN/T 2206.8-2013; the various components in the kit are dissolved on ice before use, slightly mixed and centrifuged for a short time, and then placed on ice for standby; Reagent preparation: experimental reaction system (25μL),qPCR mixed reaction solution 15.5 μL, template 1 μL, sterile ddH2O8.5μL Reaction conditions: 95 ℃ 1min 1cycle;95 ℃ 10s, 57.4 ℃ 30s 40cycle; Fluorescence Channel: FAM.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic atmosphere;
Storage conditions	-20 ℃, dark and refrigerated
Safety level	1
Separation substrate	Nail infection
application	Real-time fluorescent quantitative PCR for the detection of clinical Candida albicans.
Sharing mode	Public welfare sharing