Candida albicans nucleic acid detection kit (fluorescent PCR method)|BNCC361452 |BNCC

Essential Information Certificate Related Products

Candida albicans nucleic acid detection kit (fluorescent PCR method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Experimental preparation: before the experiment, sample enrichment culture was carried out with reference to SN/T 2206.8-2013; various components in the kit were dissolved on ice before use, slightly mixed and temporarily centrifuged, and then placed on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 15.5 μL, template 1 μL, sterile ddH2O8.5μL Reaction conditions: 95 ℃, 1min, 1cycle;95 ℃, 10s, 57.4 ℃, 30s, 40cycle; Fluorescence channel: FAM.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-20 ℃, cold storage
Safety level	1
Separation substrate	Nail infection
application	Real-time fluorescence quantitative PCR detection for clinical Candida albicans.
Sharing mode	Public welfare sharing

Candida albicans nucleic acid detection kit certificate

1. Product information

Sample name: Candida albicans nucleic acid detection kit (fluorescence PCR method)

Sample number: 361452

Sample batch: 2200505

2. Product features

Description	1.5mL cryopreservation tube package, containing reaction solution, positive quality control material, negative quality control material, DEP water
Traceability	BNCC263676(ATCC24433) Candida albicans
product specifications	1 tube of reaction solution, 1 tube of positive quality control product, 1 tube of negative quality control product, 1 tube of ddH2O water
Field of application	Real-time PCR for the detection of clinical Candida albicans
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use

3. Instructions

Experiment preparation: Before the experiment, refer to SN/T 2206.8-2013 to carry out the enrichment culture of the sample; dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly and place on ice for later use; Reagent preparation: Experimental reaction system (25 μL), 15.5 μL of qPCR mixed reaction solution, 1 μL of template, 8.5 μL of sterile ddH2O. Reaction conditions: 95°C for 1min 1cycle; 95°C for 10s, 57.4°C for 30s for 40cycle; Fluorescence channel: FAM.

4. Notes

1. The kit should be stored at low temperature in the dark, and the various components in the kit should be thawed naturally before use, mixed well and centrifuged briefly to avoid the generation of air bubbles;

2. Sample processing, reagent preparation, and sample addition should be performed in different areas to avoid cross-contamination;

3. Judgment of results: If the Ct value of the test sample is ≥38 and there is no typical amplification curve, it is judged as negative; if the Ct value of the test sample is less than or equal to 35, and two typical amplification curves appear, it is judged as positive; If the Ct value of the test sample is >35 and <38, it is recommended that the sample be redone.

5. Preservation and transportation

long-term storage:-20 ℃, valid for 1 year; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833