Bacillus subtilis quantitative strain|BNCC365155 |BNCC

BeNa Culture Collection

  • Quantitative strains of bacillus subtilis-BNCC

Quantitative strains of bacillus subtilis

  • Price: Contact
  • number:BNCC365155
  • Packing:Sylin bottle; 10 ~ 100CFU/bottle
  • Form:
    The colony is 1-2mm in diameter, round, irregular edges, opaque, gray-white front, flat surface, smooth surface, bright surface, dry texture, difficult to pick/cut, G (blue-purple), bacilli, purity: pure
Essential Information Related Products
Quantitative strains of bacillus subtilis
Culture medium Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure ① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions 37 ℃, aerobic
Growth characteristics Gram-positive, rod-shaped, rarely in chains, evenly stained; flagellum lateral, motile; endospores 0.8 × 1.5-1.8 μm, weakly colored on the surface of free spores; colonies round or irregular on the culture medium of foreign vegetables; surface color dark, opaque, can wrinkle, can become cream or brown; the shape of colonies varies greatly with the composition of the culture medium; strictly well-fed or facultative anaerobic.
Storage conditions -20 ℃
Safety level 1
morphology The colony is 1-2mm in diameter, round, irregular edges, opaque, gray-white front, flat surface, smooth surface, bright surface, dry texture, difficult to pick/cut, G (blue-purple), bacilli, purity: pure
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