BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | ① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried; |
Growth conditions | 37 ℃, aerobic |
Growth characteristics | Gram-positive, rod-shaped, rarely in chains, evenly stained; flagellum lateral, motile; endospores 0.8 × 1.5-1.8 μm, weakly colored on the surface of free spores; colonies round or irregular on the culture medium of foreign vegetables; surface color dark, opaque, can wrinkle, can become cream or brown; the shape of colonies varies greatly with the composition of the culture medium; strictly well-fed or facultative anaerobic. |
Storage conditions | -20 ℃ |
Safety level | 1 |
morphology | The colony is 1-2mm in diameter, round, irregular edges, opaque, gray-white front, flat surface, smooth surface, bright surface, dry texture, difficult to pick/cut, G (blue-purple), bacilli, purity: pure |
Sharing mode | Public welfare sharing |