Standard Quality Control Sample of Total Coliform in Drinking Water (Membrane Filter Method)|BNCC360416 |BNCC

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Standard quality control sample of total coliforms in drinking water (Membrane membrane method)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Samples required a total of 200mL of sterile water or sterile saline to dissolve. Before use, place the quality control sample at room temperature for 30min, then open it in the biosafety cabinet, immediately add 3~4mL of sterile water, suck it out after dissolution, and put it into a sterile bottle. The remaining sterile water is repeatedly washed with the inner wall of the vial, and all of it is transferred to the above sterile bottle, which is the standard quality control sample, totaling 200mL, with a concentration of 10-100CFU/100mL.
Growth conditions	37°C, aerobic, 18-24h
Storage conditions	-20 ℃, protected from light
Safety level	2
application	Specific use: Quality control samples of total coliform bacteria in drinking water (membrane method) Applicable standards: GB/T5750.12-2023-Part 12: Microbiological indicators
Sharing mode	Public welfare sharing