Vibrio parahaemolyticus (VP) and Vibrio cholerae nucleic acid detection kit (fluorescent PCR method)|BNCC361346 |BNCC

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Vibrio parahaemolyticus (VP) and Vibrio cholerae nucleic acid detection kit (Fluorescent PCR method)

Subculture procedure	1. Experimental preparation: dissolve the various components in the kit on ice before use, mix them slightly and centrifuge them briefly and place them on ice for later use; 2. Sample treatment: sample treatment shall be carried out according to SN/T 5125-2019 Inspection of Vibrio parahaemolyticus and Vibrio cholerae; 3. Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 21 μL, template 4 μL; 4. Reaction conditions: 95 ℃ 3min 1cycle;94 ℃ 10s, 50 ℃ 30s 40cycle; 5. Fluorescence channel: FAM (Vibrio parahaemolyticus), CY5 (Vibrio cholerae);
Storage conditions	-20 ℃
Safety level	0
application	It is suitable for the auxiliary diagnosis of Vibrio parahaemolyticus (VP) and Vibrio cholerae infection in water samples, stool, suspected contaminated water and food samples.
Sharing mode	Public welfare sharing