BeNa Culture Collection

  • Vibrio parahaemolyticus (VP) and Vibrio cholerae nucleic acid detection kit (fluorescent PCR method)-BNCC

Vibrio parahaemolyticus (VP) and Vibrio cholerae nucleic acid detection kit (fluorescent PCR method)

  • Price: $229
  • number:BNCC361346
  • Packing:Frozen storage tube; 25T;4 tubes/box
Essential Information Certificate Related Products
Vibrio parahaemolyticus (VP) and Vibrio cholerae nucleic acid detection kit (fluorescent PCR method)
Safety level 0
application It is suitable for the auxiliary diagnosis of Vibrio parahaemolyticus (VP) and Vibrio cholerae infection in water-like feces, suspected contaminated water, food and other samples.
Sharing mode Public welfare sharing

Vibrio parahaemolyticus and Vibrio cholerae nucleic acid test kit certificate

1. Product information

Sample name: Vibrio parahaemolyticus and Vibrio cholerae nucleic acid detection kit (fluorescent PCR method)

Sample number:  361346

Sample batch: 220420

2. Product features

Reagent composition qPCR mixed reaction solution 1 tube (525μL); Positive quality control 2 tubes (100μL); Negative quality control 1 tube (100μL)
packing specifications 25 T/box
sensitivity 1.87copies/μL
applicable fields It is suitable for auxiliary diagnosis of Vibrio parahaemolyticus and Vibrio cholerae infection in water-like feces, suspected contaminated water, food and other samples
save and transport -20 ℃, valid for 1 year, dry ice transportation
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use

3. Instruction

1. Experimental preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, and place on ice for later use;

2. Sample processing: sample processing according to "SN/T 5125-2019 Inspection of Vibrio parahaemolyticus and Vibrio cholerae";

3. Reagent preparation: experimental reaction system (25 μL), 21 μL qPCR mixed reaction solution, 4 μL template;

4. Reaction conditions: 95°C 3min 1cycle; 94°C 10s, 50°C 30s 40cycle;

5. Fluorescence channel: FAM (Vibrio parahaemolyticus), CY5 (Vibrio cholerae);

4. Result judgment

1. The sample has a typical amplification curve, and the Ct value is less than or equal to 35;

2. The sample has no amplification curve, and the Ct value is ≥40 or not detected;

3. If the t value of the sample is >35 and <40, it needs to be re-measured. Retest results Ct value ≥ 40 is negative, Ct value < 40 is positive.

5.Preservation and transportation

1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;

2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

Henan Engineering Research Center of Industrial Microbiology

Website: www.bncc .org .cn Tel: 400-6699-833

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