BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | When using essential control products, they should be moved to room temperature (20~25 ℃) for 10 minutes, dissolved with 1mL sterile water or sterile physiological saline, with a minimum sampling volume of 200uL, and counted with 100 μg/mL canamycin LB plate, with a concentration value of 108 ~ 109CFU/mL and a reference concentration of 1.5 × 108CFU/mL. |
Growth conditions | 37 ℃,18-24h, aerobic |
Storage conditions | -20 ℃ |
Safety level | 2 |
application | sfgfp, also known as enhanced green fluorescent protein, can emit green fluorescence under blue light excitation, with stable chemical properties, almost no toxicity to cells, and easy to detect. The green fluorescence characteristics of sfgfp were introduced into Salmonella typhimurium as tracer bacteria, and its fluorescence properties could be used to detect Salmonella typhimurium. |
Sharing mode | Public welfare sharing |