Standard substance for enumeration of Staphylococcus aureus in food|BNCC359095 |BNCC

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Reference material of staphylococcus aureus count in food

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① Positive quality control bacteria:; Negative quality control bacteria:; ③ Activate and increase bacteria according to the above quality control sample, so that the absorbance of ten times dilution of the two bacteria is in the range of 0.3~0.5; ④ Mix the two original bacteria solutions in equal volume, dilute 25000 times and then suck 0.5mL of final diluent into 10mL of protective agent (quantitative bacteria protective agent), and mix well; ⑤ Sub-pack the mixed solution into 0.4mL/vial, pre-freeze it at -80 ℃ for 20-24 hours, take it out and freeze it overnight, and cap the freeze-dried quality control product and label it for storage the next day;
Growth conditions	37°C, aerobic, 18-24h
Storage conditions	-20 ℃, protected from light
Safety level	2
application	This standard substance can be used for the method verification of quantitative detection of Staphylococcus aureus in food and the quality control of microbiological laboratory measurement, and can also be used for the growth rate evaluation of Staphylococcus aureus selective medium, which provides measurement traceability guarantee for the reliability of the evaluation results.
Sharing mode	Public welfare sharing