Escherichia coli quantitative strain|BNCC356331 |BNCC

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Quantitative strains of escherichia coli

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃;18-24h; aerobic;
Growth characteristics	G-bacilli, positive for lysine decarboxylase and ornithine decarboxylase, negative for arginine dihydrolase, negative for urease and phenylalanine deaminase, IMViC test: +, +,-,-; positive for MUG, negative for cellobiose, negative for lateral marigold, positive for ammonium glucose; no H2S production, fermentation of lactose to produce acid and gas. facultative anaerobic, optimum pH7.4 ~ 7.6.
Storage conditions	-20 ℃
Safety level	1
morphology	The colony is 1-2mm in diameter, round, with neat edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, smooth surface, bright surface, moist texture, easy to provoke, G-(red), bacillus, purity: pure
application	research; analysis and detection. Research, quality control bacteria, "GB 4789.2-2010 colony count determination", "GB 4789.3-2010 coliform count", research; analysis and detection. GB/T 4789.32-2002 coliform rapid test, GB 4789.39-2013 fecal coliform count positive control strains, the 2015 version of the Chinese Pharmacopoeia quality control strains.
Sharing mode	Public welfare sharing