Seawater Fecal Coliform Quality Control Sample (Multi-tube Fermentation Method)|BNCC363179 |BNCC

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Serwater fecal coliform positive control quality control sample (Multi-tube fermentation method)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Samples required a total of 100 mL of sterile water or sterile saline to dissolve. Before use, place the quality control sample at room temperature for 30 min, then open it in the biosafety cabinet, immediately add 3-4 mL of sterile water, dissolve it, suck it out and put it into a sterile bottle, repeatedly wash the inner wall of the vial with the remaining sterile water, and transfer it to the above sterile bottle, which is the quality control sample suspension, totaling 100 mL, with a concentration of 800-8000 MPN/L.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic atmosphere;
Storage conditions	-20 ℃
Safety level	0
application	It is applicable to the detection, quality control and personnel assessment of marine fecal coliform in the standard of GB 17378.7-2007 Marine Monitoring Specification-Part 7: Ecological Investigation and Biological Monitoring of Offshore Pollution.
Sharing mode	Public welfare sharing