Mycobacterium chelonae DNA lab quality control|BNCC364926 |BNCC

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Mycobacterium chelonae DNA lab quality control

Culture medium	Ammonium sulfate: 0.5, potassium dihydrogen phosphate: 1.5, disodium hydrogen phosphate: 1.5, sodium citrate: 0.4, magnesium sulfate: 0.025, calcium chloride: 0.5mg, zinc sulfate: 0.001, copper sulfate: 0.001, sodium L-glutamate: 0.5, iron ammonium citrate: 0.04, pyridoxine hydrochloride: 0.001, biotin: 0.5mg, malachite green: 0.25mg, agar: 15.0,PH:6.4-6.8(25 ℃)
Subculture procedure	When using this standard, it is moved to room temperature (20~25 ℃) and then thawed, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, trying to avoid repeated freezing and thawing.
Growth conditions	30 ℃;48-72h; aerobic;
Storage conditions	-80 ℃
Safety level	2
application	The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated suspension of the turtle mycobacterium, which can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc. by adding stabilizer and digital PCR fixed value.
Sharing mode	Public welfare sharing