Staphylococcus aureus quantitative strains|BNCC353786 |BNCC

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Quantitative strains of staphylococcus aureus subsp. aureus Rosenbach

Culture medium	Tryptone: 17.0, soy peptone: 3.0, sodium chloride: 5.0, dipotassium hydrogen phosphate: 2.5, glucose: 2.5g,pH:7.3±0.2(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃;18-24h; aerobic;
Storage conditions	-80 ℃
Safety level	2
morphology	The colony is 1-2mm in diameter, round, with neat edges, opaque, gray-white front, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, G + (blue-purple), cocci, purity: pure
Separation substrate	Human lesion
application	It is mainly used for medium performance testing, positive control, laboratory ability verification, personnel ability verification, laboratory quality control and method evaluation.
Sharing mode	Public welfare sharing