BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | Samples required a total of 100mL of sterile water or sterile saline to dissolve. Before use, place the quality control sample at room temperature for 30min, then open it in the biosafety cabinet, immediately add 3~4mL of sterile water, suck it out after dissolution, and put it into a sterile bottle. The remaining sterile water is repeatedly washed with the inner wall of the vial, and all of it is transferred to the above sterile bottle, which is the standard quality control sample, totaling 100mL, with a concentration of 10-100CFU/100mL. |
Growth conditions | 37°C, aerobic, 18-24h |
Storage conditions | -20 ℃, protected from light |
Safety level | 2 |
application | Specific use: drinking water Escherichia coli quality control samples (membrane method) Applicable standards: GB/T5750.12-2023 "drinking water standard test methods part 12: microbial indicators" |
Sharing mode | Public welfare sharing |