Yersinia enterocolitica DNA standard|BNCC363503 |BNCC

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Yersinia enterocolitica DNA standard

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	When this standard is used, it is moved to room temperature to be completely dissolved, shaken evenly, and used after instantaneous centrifugation to avoid repeated freezing and thawing as much as possible.
Growth conditions	37 ℃;18-24h; Aerobic;
Storage conditions	-80 ℃
Safety level	1
application	This Yersinia enterocolitica DNA standard, through fluorescence quantitative pcr combined with digital PCR, can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing