QUANTITATIVE QUANTITATIVE QUANTITATIVE QUANTITATIVE STRAINS OF ESCHERICHIA COLI (ENZYME SUBSTRATE METHOD)|BNCC370600 |BNCC

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Escherichia coli quality control sample

Culture medium	Ammonium sulfate: 5.0, manganese sulfate: 0.0005, zinc sulfate: 0.0005, magnesium sulfate: 0.1, sodium chloride: 10.0, calcium chloride: 0.05, sodium sulfite: 0.04, amphotericin B:0.001,ONPG:0.5,MUG:0.075,Solanium extract: 0.5,HEPES:6.9,HEPES sodium salt: 5.3
Subculture procedure	Before use, the quality control sample is placed at room temperature for 30min, and the sample needs a total of 100mL of sterile water or sterile physiological saline to dissolve. Then open it in the biosafety cabinet, immediately add 3~4mL of sterile water, suck it out after dissolution and put it into a sterile bottle, repeatedly wash the inner wall of the vial with the remaining sterile water, and transfer it to the above sterile bottle, which is 100mL standard sample stock solution.
Growth conditions	37 ℃, aerobic; culture time 24-48 hours; gas environment anaerobic
Storage conditions	-20 ℃, protected from light
Safety level	1
application	Reference standard: HJ 1001-2018 Enzyme substrate method for determination of total coliform, fecal coliform and Escherichia coli in water quality; Positive control for determination of total coliform, fecal coliform and Escherichia coli in water quality (enzyme substrate method).
Sharing mode	Public welfare sharing