NRS 1221A quantitative strain of Bacillus atrophy|BNCC359607 |BNCC

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NRS 1221A quantitative strain of Bacillus atrophy

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Growth conditions	30 ℃;18-24h; Aerobic;
Storage conditions	-20 ℃
application	Resistance Testing Dry heat sterilization control Efficacy testing Ethylene oxide sterilization control Media testing Steam sterilization control Sterility assurance Sterilization control Pharmaceutical and Personal Care Resistance test dry heat sterilization control efficacy test ethylene oxide sterilization control medium inspection BR> sterility assurance sterilization control> drugs and personal care
Sharing mode	Public welfare sharing

Bacillus atrophaeus Nakamura

Strain number: BNCC 359607 / ATCC 9372

Product format: quantitative bacteria tablets

Product batch:2021.08.26

Date of inspection:2021.08.27

Valid until:2022.08.25

Number of viable bacteria:50-100CFU/bottle

Sampling ratio:≥2%

Report number:RS21359607- 1

Biosafety level: 1, handle in ultra-clear table or safety cabinet

items	test results	description
stain microscopy	gram negative and positive, cell morphology observed under microscope	G + bacilli
colony morphology	observation of single colony morphology	reddish brown, flat, irregular edges
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
Reference value of live bacteria	Non-selective medium plate, viable count	80CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list:1 bottle of 50-100CFU quantitative strain, 1 bottle of 1mL lysate, 1 strain certificate;

Validity period: unopened, below -20 ℃, 12 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After fully dissolved, it is 50- 100CFU/mL, which is absorbed in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. Improper storage conditions and use methods will affect the accuracy of the number of strains, so please operate in strict accordance with the above storage conditions and methods.

②Used germ-carrying consumables should be discarded after autoclaving.