Azotobacter sp.|BNCC335805 |BNCC

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Azotobacter sp.

Culture medium	Rhizobium YMA medium (YMA):KH2PO4 0.25g,K2HPO4 0.25g,MgSO4 · 7H2O 0.2g,NaCl 0.1g, yeast extract 0.8g, mannitol 10.0g, agar 18.0g, distilled water 1.0 L,pH 7.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;24-48h; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: 2-4mm Shape: round Edge: neat Transparency: Transparency Color: colorless and transparent Protuberance: Middle bulge Surface: bright and smooth Texture: moist and viscous
application	Bacterial fertilizer
Sharing mode	Public welfare sharing

1.Description

1. Name: Azotobacter sp.

2. BNCC No.: 335805

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Medium composition:KH2PO4 0.2g，K2HPO4 0.8g，MgSO4.7H2O 0.2g，CaSO4·2H2O 0.1g，Na2MoO4.2H2O trace, yeast extract 0.5g, mannitol 20g,FECL3 Tract trace, distilled water 1000ml, agar 15g,PH7.2

2. Culture time: 24-48h

3, Temperature: 28-30 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization