Bacteroides caccae Johnson et al.|BNCC232292 |BNCC

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Bacteroides caccae Johnson et al.

Culture medium	Improved GAM medium (mGAM): peptone 5.0g,䏡 Peptone 5.0g, soybean peptone 3.0g, yeast powder 2.5g, beef powder 2.2g, digestive serum powder 10.0g, beef liver powder 1.2g, glucose 0.5g, potassium dihydrogen phosphate 2.5g, sodium chloride 3.0g, soluble starch 5.0g,L-cysteine 0.3g,L-arginine 1.0g,L-tryptophan 0.2g, sodium thioglycolate 0.3g,PH 7.3±0.1,121 ℃,15min. note: (add 1ml of sterile 0.1% vitamin K1 solution and 2mg of sterile hemin for every 200ml, and mix well). preparation of sterile hemin solution: weigh appropriate amount of hemin, add appropriate amount of distilled water, and sterilize under high pressure at 116 ℃ for 20-30 minutes.
Subculture procedure	(1) Prepare 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions.
Growth conditions	37 ℃,24-48h, anaerobic
Storage conditions	2-8 ℃
morphology	Round, neat edges, opaque, light color, light color, convex in the middle, smooth and bright surface, moist texture, easy to pick up, no pigment, G-(red), bacilli, purity: pure
Separation substrate	Human feces, Texas
application	Characterization
Sharing mode	Public welfare sharing

Bacteroides caccae Johnson et al.

Storage conditions: 2~8 ℃

No. 232292

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, strictly anaerobic, improved GAM agar: 15.0g of monthly peptone, 10.0g of tryptone, 3.0g of soybean peptone, 5.0g of yeast powder, 2.0g of beef powder, 1.2g of beef liver powder, 0.3g of soluble starch, 0.15g of L-cysteine, 3.0g of glucose and 2.5g of potassium dihydrogen phosphate, digested serum powder 13.5g, sodium thioglycolate 0.15g, sodium chloride 3.0g, agar 13.0g, distilled water 1L,pH 7.0±0.2, 25 ℃ additive: 0.02% vitamin K1 solution and hemin

Recovery steps:
(1) Prepare 2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water(keep in an anaerobic environment for 24 hours) into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 5-7 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 48h strain layer is obvious; colony is typical on the streaked plate
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Translucent color: light yellow uplift: middle convex surface: bright and smooth texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified