purple silk membrane bacteria|BNCC230932 |BNCC

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Cortinarius rufo-olivaceus

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of PDA plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions for 5-7 days.
Growth conditions	Culture temperature 28 ℃; Culture time 5-7 days; Aerobic atmosphere;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, obviously visible on integrated PDA agar medium, colonies pink-white, dry, vigorous, in culture The base surface spreads and does not produce spores.
Sharing mode	Public welfare sharing

Cortinarius rufo-olivaceus

BNCC No.：230932

Storage conditions: 2~8 ℃

Product format: agar slant in 14mm test tube

Validity period: 30 days

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:25-28 ℃, aerobic, PDA. PDA: potato extract 6g, glucose 20g, agar 20g,pH natural, 121 ℃,15min.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4) Lay flat the small pieces to the center of the agar plate;

(5)Put the agar plates under the above culture conditions for cultivation 5-7days

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 7 days plate colony is obvious
colony morphology:	small filamentous fungi are obviously visible on integrated PDA agar medium, colonies are pink-white, dry, vigorous, spread on the surface of the culture medium and do not produce spores.
conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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