BeNa Culture Collection
Culture medium | Peptone: 10.0, beef extract powder: 8.0, yeast powder: 4.0, glucose: 20.0, magnesium sulfate: 0.2, sodium acetate: 5.0, diammonium hydrogen citrate: 2.0, dipotassium hydrogen phosphate: 2.0, manganese sulfate: 0.04, Tween 80:1.0, agar: 14.0,pH:5.7±0.2(25 ℃) |
Subculture procedure | The standard inoculum was inoculated into esculin, cellobiose, maltose, mannitol, salicin, sorbitol, sucrose and raffinose vials respectively (37 ℃ after inoculation, full plug culture for 24-48h). Please refer to the GB4789.35-2016 standard to judge the results. |
Growth conditions | The culture temperature is 37 ℃; the culture time is 24-48 hours; the gas environment is microaerobic (3%-5% O2,10% CO2); |
Storage conditions | 2-8 ℃ |
Safety level | 0 |
application | It is suitable for the physiological and biochemical identification of Lactobacillus in GB4789.35-2016 food microbiological examination of lactic acid bacteria. |
Sharing mode | Public welfare sharing |