Enterobacter sakazakii biochemical identification kit|BNCC361930 |BNCC

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Culture medium	Tryptone: 17.0, soy peptone: 3.0, sodium chloride: 5.0, dipotassium hydrogen phosphate: 2.5, glucose: 2.5g,pH:7.3±0.2(25 ℃)
Subculture procedure	The yellow suspicious colonies were directly picked from TSA plate and inoculated with L-lysine decarboxylase broth (1), L-ornithine decarboxylase broth (2), L-arginine dihydrolase (3), amino acid decarboxylase control (4), Simon's citrate (5), D-sorbitol (6), L-rhamnose (7), D-sucrose (8), D-honey disaccharide (9), amygdalin (10), oxidase test (11). Please refer to the GB4789.40-2016 standard to judge the result. (After inoculation, put 30 ℃ (Simon's citrate 37 ℃, semi-plug) and culture with full plug for 24±2h, and add sterilized paraffin to cover the liquid level of the medium after inoculation in the amino acid decarboxylase control tube)
Growth conditions	Culture temperature 30 ℃;18-24 hours of culture time; Aerobic gas environment;
Storage conditions	2-8 ℃
Safety level	0
application	Physiological and biochemical identification of Enterobacter sakazakii in "GB4789.40-2016 food microbiological examination of Cronobacter (Enterobacter sakazakii)"
Sharing mode	Public welfare sharing