Staphylococcus saprophyticus nucleic acid detection kit (fluorescent PCR method)|BNCC372864 |BNCC

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Culture medium	Bovine brain extract powder: 4.0, bovine heart extract powder: 4.0, peptone: 5.0, casein peptone: 16.0, sodium chloride: 5.0, glucose: 2.0, disodium hydrogen phosphate: 2.5, agar: 13.5,pH:7.4±0.2(25 ℃)
Subculture procedure	1. Experimental preparation: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Sample processing: After the positive quality control is dissolved, it is then placed on ice for later use; 3. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 2 μL of template and 8 μL of sterile ultrapure water, mix well and centrifuge for a short time; 4. Reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 53 ℃ for 30s, and the second to third steps are cycled 40 times. 5. Fluorescence channel: FAM;
Growth conditions	37 ℃;24-48h; aerobic
Storage conditions	-20 ℃
Safety level	1
application	Rapid detection of Staphylococcus saprophyticus
Sharing mode	Public welfare sharing