Human adenovirus type 41 (ADV41) nucleic acid detection kit (fluorescent PCR method)|BNCC361556 |BNCC

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Human adenovirus 41 nucleic acid detection kit (QPCR)

Culture medium	98%EMEM+2%FBS
Subculture procedure	Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 17 μL, template 2 μL, sterile ddH2O to 25 μL; Experimental reaction conditions: the first step 95 ℃ 1min, the second step 95 ℃ 10s, the third step 60 ℃ 30s, the second to third steps cycle 40 times; Fluorescence Channel: FAM.
Growth conditions	Culture temperature 37 ℃; Culture time 5 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-20 ℃, protected from light
Safety level	1
application	It is used for the detection of specific in vitro amplification of DNA of human adenovirus type 41 by fluorescence PCR technology, which can not be used for the diagnosis of clinical diseases.
Sharing mode	Public welfare sharing