BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | After taking out the standard sample from the refrigerator at -20 ℃, equilibrating at (20±2)℃ for 10 min, opening it in the biosafety cabinet, adding 5mL of sterile water or sterile physiological saline to redissolve the freeze-dried quality control substance, fully mixing and then preparing (to be tested) standard sample suspension with a concentration of (1.9±0.4)× 10 5CFU/mL |
Growth conditions | 37°C, aerobic, 18-24h; |
Storage conditions | -20 ℃, dark and refrigerated; ice bag transportation |
Safety level | 2 |
application | "GB/T 5750.12-2023 Standard Test Methods for Drinking Water Microbial Indicators Total Colony Plate Counting Method" and "HJ 1000-2018 Determination of Total Bacteria in Water Quality Plate Counting Method" The strain numbers required for production are: BNCC269342, BNCC286903, BNCC238481 and BNCC293727. |
Sharing mode | Public welfare sharing |