Standard substance for enumeration of Escherichia coli in food|BNCC359092 |BNCC

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Reference material of escherichia coli count in food

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Positive quality control bacteria: BNCC269342 ATCC25922; ② Negative quality control bacteria: none; ③ Activate and increase bacteria according to the above quality control sample, so that the absorbance of ten times dilution of the two bacteria is in the range of 0.3~0.5; ④ Mix the two original bacteria solutions in equal volume, dilute 25000 times and then suck 0.5mL of final diluent into 10mL of protective agent (quantitative bacteria protective agent), and mix well; ⑤ Sub-pack the mixed solution into 0.4mL/vial, pre-freeze it at -80 ℃ for 20-24 hours, take it out and freeze it overnight, and cap the freeze-dried quality control product and label it for storage the next day;
Growth conditions	37 ℃, aerobic, 18-24h
Storage conditions	-20 ℃, protected from light
Safety level	1
application	Sensitivity test; Bacteriostasis test; Food detection; Cosmetic detection; Culture control; Quality control strain; API verification bacteria, positive control strain in GB 4789.38-2012 Escherichia coli count.
Sharing mode	Public welfare sharing