Escherichia coli quantitative strain|BNCC354967 |BNCC

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Quantitative strains of Escherichia coli

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	1. Open the strain and solution according to the arrow mark of the Xilin bottle cap; 2. Pour the bacteria into the dissolved liquid, cover the rubber plug, and shake to dissolve; 3. After fully dissolved, according to the test requirements according to the proportion of absorption can be; 4. Please use up immediately after opening, and shall not be stored;
Growth conditions	37 ℃;18-24h; aerobic;
Growth characteristics	G-bacilli, positive for lysine decarboxylase and ornithine decarboxylase, negative for arginine dihydrolase, negative for urease and phenylalanine deaminase, IMViC test: +, +,-,-; positive for MUG, negative for cellobiose, negative for lateral marigold, positive for ammonium glucose; no H2S production, fermentation of lactose to produce acid and gas. facultative anaerobic, optimum pH7.4 ~ 7.6.
Storage conditions	-20 ℃
Safety level	1
morphology	Light yellow, raised, moist, bright, G-bacillus
application	research; analysis and detection. Research, quality control bacteria, "GB 4789.2-2010 colony count determination", "GB 4789.3-2010 coliform count", research; analysis and detection. GB/T 4789.32-2002 coliform rapid test, GB 4789.39-2013 fecal coliform count positive control strains, the 2015 version of the Chinese Pharmacopoeia quality control strains.
Sharing mode	Public welfare sharing