BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | When using the essential control substance, it should be moved to room temperature (20~25 ℃) to balance for 10 minutes, the concentration value is 10 ^ 8~10 ^ 9PFU/mL, and the reference concentration is 8.5 × 10 ^ 8PFU/mL |
Safety level | 1 |
application | The E. coli phage PhiX174 quantitative standard quality control, by cultivating E. coli host bacteria and phage PhiX174, collect phage. The titer and lysis efficiency of the collected phage were determined by double-layer agar counting method, which was mainly used for the quality control of phage PhiX174 positive. |
Sharing mode | Public welfare sharing |