BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | When using essential control products, they should be moved to room temperature (20~25 ℃) and balanced for 10 minutes, with a concentration of 10 ^ 6~10 ^ 7PFU/mL and a reference concentration of 9.33 × 10 ^ 6PFU/mL. |
Storage conditions | -20 ℃ |
Safety level | 1 |
application | The E. coli phage PhiX174 quantitative standard quality control, by cultivating E. coli host bacteria and phage PhiX174, collect phage. The titer and lysis efficiency of the collected phage were determined by double-layer agar counting method, which was mainly used for the quality control of phage PhiX174 positive. |
Sharing mode | Public welfare sharing |